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nanodrop nd-1000 spectrophotometer
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The NanoDrop ND-1000 Spectrophotometer is capable of full spectrum UV-Vis absorbance analyses (220-750nm) using 1µL of sample. Its patented sample retention technology uses fiber optic connectors to hold the sample in place during measurement. For dsDNA, the concentration range of the sample should be 2-3700ng/µL for accurate quantification.

Unlike traditional spectrophotometers, the Nanodrop does not require cuvettes or capillaries. Instead, the sample is pipetted directly onto the measurement pedestal. When the apparatus is closed, the sample arm compresses the droplet and a sample column is drawn (to a controlled path length of 1mm) and surface tension holds the sample in place. After the 10 second measurement, the sample is simply wiped off and the instrument is ready for the next sample. The accuarcy of the measurement is typically within 2%.

To learn more, visit the Nanodrop website.

Cost: $5 per sample
Turnaround time: 2 business days


Download the Nanodrop Spectrophotometer Checklist and Form

Please note that samples for the NanoDrop Spectrophotometer should be clearly labelled with unique names.

Quick questions:
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What is the minimum amount (volume and concentration) of sample that can be read?

For nucleic acids, the minimum volume required is 1µL and the concentration should be between 2-3700 ng/µL.
For protein samples, 2 µL of sample is required.

Customers are asked to bring/submit at least 2 µL for nucleic acid quantitation and 4 µL for protein quantitation.

Is it possible to retrieve the sample after it’s been measured?

No, the sample cannot be retrieved following its measurement.

What wavelengths can be read?

The Nanodrop ND-1000 spectrophotometer is able to perform full spectrum UV-Vis absorbance analyses (220-750nm). Using the microarray application, the concentration of nucleic acid and up to 2 dyes can be calculated and displayed at the same time. back to top

Are there solvent restrictions for the Nanodrop?

There are a few solvent restrictions when using the Nanodrop. For example, hydrofluoric acid should definitely not be used. Samples dissolved in high vapour pressure solvents such as acetone can be measured but only using the short path length to reduce evaporation. Please contact us prior to using the Nanodrop if you are using non-standard buffers/solvents.