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validation technology comparison
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The UHNMAC offers several technologies for validating the data obtained from high-density microarray experiment: Bio-Plex, Nanostring and Ziplex. These three new technologies enable highly-multiplexed microarray data validation.

Quantitative PCR is considered the gold standard technique for validating microarray results, although this technique can be labourious and costly if you have a large number of genes or samples that require validation.
Please note that we do not offer a qPCR service.

qPCR Bio-Plex Nanostring Ziplex (Xceed)
Advantages Gold standard
TaqMan probes available for most genes
Highly multiplexed
Also capable of protein assays
No enzymology
Highly multiplexed
Array-based method (similar protocol and data format to high-density arrays)
# genes requiring validation < 20 20-80 30-500 (and for >96 different samples, or multiples of 12 if using catalog CodeSets) 20-120
Theoretical maximum # of genes per sample (per run), including controls 4 100 550 400
Practical # of genes per sample (per run) 1 80 500 120
Amount of total
RNA required
1-100 ng coming soon 100 ng 500 ng
Detection limit <1 copy per cell
(<0.5 fM)
coming soon <1 copy per cell
(<0.5 fM)
approx. 400 copies per cell (0.2 pM)
Dynamic range 2 logs (100-fold) coming soon 3 logs
(but as high as 6 logs)
3 logs
Works with
degraded RNA?
No Good results from FFPE samples provided >50% had length > 300 bp
# different samples processed simultaneously (per run) 96 or 384 96 12 8
Time per run 5 hours coming soon 18 hours (including 12 hour overnight hyb) 3 hours + approx. 20 hours for IVT reactio